other:Article Title: The mechanosensitive ion channel Piezo1 modulates the migration and immune response of microglia.
Article Snippet: Antibodies Iba1 rabbit monoclonal primary antibody Cell Signaling Technology Cat. # 17198S; RRID: AB_2820254 Iba1 goat polyclonal primary antibody Novus Biologicals Cat. # NB100-1028 Piezo1 rabbit polyclonal primary antibody Proteintech Cat. # 15939-1-AP; RRID: AB_2231460 Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo-Fisher Scientific Cat. # A-11008; RRID: AB_143165 Anti-IL-1 beta primary antibody Abcam Cat. # ab254360 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo-Fisher Scientific Cat. # 31460; RRID: AB_228341
Recombinant:
Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
Article Title: Gasdermin-E mediates mitochondrial damage in axons and neurodegeneration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (Cat#; RRID) Antibodies Rabbit monoclonal anti-GSDME Abcam #ab215191; RRID:AB_2737000 Rabbit monoclonal anti-GSDMD Abcam #ab209845; RRID:AB_2783550 Rabbit monoclonal anti-Caspase-3 Cell Signaling #9662S; RRID:AB_331439 Mouse monoclonal anti-GAPDH EMD Millipore #CB1001; RRID:AB_2107426 Rabbit monoclonal anti-Cytochrome-C Cell Signaling #11940S; RRID:AB_2637071 Mouse monoclonal anti-ATPF1 Abcam #ab117991; RRID:AB_10901555 Mouse monoclonal anti- ATP5A Abcam #ab14748; RRID:AB_301447 Rabbit polyclonal anti-FLAG Sigma #F7425-.2MG; RRID:AB_439687 Mouse monoclonal anti-Tuj1 Thermo Scientific #MA1-118X; RRID:AB_2536830 Mouse monoclonal anti-GFAP Cell Signaling #3670S; RRID:AB_561049 Rabbit monoclonal anti-Iba1 Cell Signaling #17198S; RRID:AB_2820254 Rabbit monoclonal anti-CD68 Cell Signaling #97778S; RRID:AB_2928056 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling #7074; RRID:AB_2099233 Biotinylated anti-Rb IgG secondary antibody Vector #BA-1000; RRID:AB_2313606 Rabbit monoclonal anti-Isl1/2 Abcam #ab109517; RRID:AB_10866454 Biological samples Sections of human brain tissue, see Table S1 UPenn Center for Neurodegenerative Disease Research Biobank NA Chemicals, peptides, and recombinant proteins Poly-ornithine Sigma #P3655-50mg Laminin Life Technologies #23017-015 Matrigel Basement Membrane Matrix Corning #354234 Neurobasal Medium Gibco #21103 Glutamax Gibco #35050 Dextrose Sigma #D9434-250g MEM NEAA Invitrogen #11140-050 B27 Life Technologies #17504-044 BDNF Peprotech #450-02 GDNF Peprotech #450-13 CNTF Peprotech #450-10 Puromycin Life Technologies #A11138-03 Doxycycline hyclate Sigma #D9891-5g ROCKi (Y-27632) Stemcell Technologies #72304 Raptinal Sigma #SML1745 (Continued on next page) Neuron 111, 1222–1240.e1–e9, April 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER (Cat#; RRID) Rotenone Sigma #R8875 Antimycin-A Sigma #A8674 3-NP Sigma #N5636 T-per buffer Thermo #78510 tablet protease inhibitor Sigma #11836153001 HALT protease inhibitor Thermo #87786 1 tablet Phosstop - phosphatase inhibitor tablets Sigma/Roche #04906845001 RIPA lysis buffer EMD Milipore #20-188
Article Title: CLN3 mediates chloride efflux from lysosomes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal anti-TFEB Abcam Cat# ab267351; RRID:AB_3674319 polyclonal anti-TFEB Cell Signaling Technology Cat# 4240S; RRID:AB_11220225 monoclonal anti-alpha-tubulin Thermo Fisher Scientific Cat# A11126; RRID:AB_10563441 monoclonal anti-ATP synthase C Abcam Cat# ab181243; RRID:AB_2935765 polyclonal anti-p62 MBL Cat# PM045; RRID:AB_1279301 polyclonal anti-Iba1 FUJIFILM Wako Cat# 019-19741; RRID:AB_839504 monoclonal anti-Iba1 Cell Signaling Technology Cat# 17198S; RRID:AB_2820254 polyclonal anti-GFAP Millipore Cat# AB5541; RRID:AB_177521 monoclonal anti-GFAP Cell Signaling Technology Cat# 3670S; RRID:AB_561049 monoclonal anti-Lamp2 Abcam Cat# AB13524; RRID:AB_2134736 Donkey anti-rabbit Alexa Fluor 647 Thermo Fisher Scientific Cat# A31573; RRID:AB_2536183 Donkey anti-mouse Cy3 Jackson ImmunoResearch Cat# 715-165-151; RRID:AB_2315777 Donkey anti-chicken Alexa Fluor 488 Jackson ImmunoResearch Cat# 703-545-155; RRID:AB_2340375 IRDye® 680RD Goat anti-rabbit IgG (H+L) secondary antibody LI-COR Cat# 926-68071; RRID:AB_10956166 IRDye® 800CW Goat anti-mouse IgG (H+L) secondary antibody LI-COR Cat# 926-32210; RRID:AB_621842 Bacterial and virus strains NEB® Stable Competent E. coli New England Biolabs Cat# C3040H DH5α Competent Cells This paper N/A Chemicals, peptides, and recombinant proteins Hydrochloric acid (HCl) Sigma-Aldrich Cat# 7647-01-0 Methanesulfonic acid (MSA) Sigma-Aldrich Cat# 471356 N-methyl-D-glucamine (NMDG) Sigma-Aldrich Cat# M2004 EGTA Sigma-Aldrich Cat# E4378 HEPES Sigma-Aldrich Cat# H3375 MES hydrate Sigma-Aldrich Cat# M8250 Glucose Sigma-Aldrich Cat# G6152 K-Gluconate Sigma-Aldrich Cat# P1847 KCl Sigma-Aldrich Cat# P9541 NaCl Sigma-Aldrich Cat# S9888 CaCl 2 Sigma-Aldrich Cat# C8106 MgCl 2 Sigma-Aldrich Cat# 208337 NaOH Sigma-Aldrich Cat# 567530 KNO 3 Sigma-Aldrich Cat# 221295 NaNO 3 Fisher Scientific Cat# S343 Ca(NO 3 ) 2 Sigma-Aldrich Cat# C1396 Mg(NO 3 ) 2 Sigma-Aldrich Cat# 237175 NaBr Sigma-Aldrich Cat# 310506 NaI Sigma-Aldrich Cat# 383112 NaH 2 PO 4 Sigma-Aldrich Cat# S3139 K 2 HPO 4 Sigma-Aldrich Cat# P3786 MgSO 4 Sigma-Aldrich Cat# M7506 Ethanol Sigma-Aldrich Cat# 459844 (Continued on next page) Neuron 114, 1–16.e1–e7, March 4, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Fisher Scientific Cat# A452SK-4 2-propanol Sigma-Aldrich Cat# 1.02781 Triton-X 100 Sigma-Aldrich Cat# X100 isoflurane Dechra N/A DMEM Thermo Fisher Scientific Cat# 10566016 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5670701 TrypLETM express enzyme (1X), no phenol red Thermo Fisher Scientific Cat# 12604039 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 MEM amino acids (50x) solution Sigma-Aldrich Cat# M5550 HBSS, no calcium, no magnesium, no phenol red Thermo Fisher Scientific Cat# 14175095 JetOPTIMUS DNA transfection reagent Polyplus Cat# 101000006 Polybrene Sigma-Aldrich Cat# H9268 Puromycin Sigma-Aldrich Cat# P8833 Poly-L-lysine hydrobromide Sigma Aldrich Cat# P1399 Vacuolin-1 Sigma Aldrich Cat# 673000 Nigericin (sodium salt) MedChemExpress (MCE) Cat# HY-100381 Valinomycin Sigma Aldrich Cat# 94675 Tributyltin chloride Sigma Aldrich Cat# T50202 Monensin sodium salt MedChemExpress (MCE) Cat# HY-N0150 Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat# D8418 DIDS Tocris Bioscience Cat# 4523 Niflumic acid Sigma Aldrich Cat# N0630 5-Nitro-2-(3phenylpropylamino)benzoic acid Sigma Aldrich Cat# N4779 Curcumin analog C1 SelleckChem Cat# S6769 Eltrombopag MedChemExpress (MCE) Cat# HY-15306 DTT Thermo Fisher Scientific Cat# P2325 Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1600-100 Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-121 Protease inhibitor cocktail Sigma Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail I Sigma Aldrich Cat# 4906845001 LDS sample buffer (4x) Thermo Fisher Scientific Cat# NP0008 PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# 23227 Intercept® (TBS) Blocking Buffer LI-COR Cat# 927-60001 20xTBS TweenTM-20 Buffer Thermo Fisher Scientific Cat# 28360 PVDF membrane Sigma Aldrich Cat# IPFL00010 TRIzolTM Reagent Thermo Fisher Scientific Cat# 15596026 RIPA This paper N/A LysoSensorTM Green DND-189 Thermo Fisher Scientific Cat# L7535 Dextran, Oregon GreenTM 488 Thermo Fisher Scientific Cat# D7170 Magic Red Cathepsin L Immunochemistry Cat# 941 DQTM-BSA red dye Thermo Fisher Scientific Cat# D12051 Dil Thermo Fisher Scientific Cat# D282 Critical commercial assays Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S GoTaq® G2 Green Master Mix Promega Cat# M7822 ClonExpress II One Step Cloning Kit Vazyme Cat# C112 2 × Phanta Flash Master Mix (Dye Plus) Vazyme Cat# P520 (Continued on next page) e2 Neuron 114, 1–16.e1–e7, March 4, 2026
Reverse Transcription:
Polymerase Chain Reaction:
Real-time Polymerase Chain Reaction:
Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
SYBR Green Assay:
Immunohistochemistry:
Polymer:
RNA Extraction:Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
BIA-KA:Article Title: A strategy of microglia replacement alleviates microgliopathy in a CSF1R I794T hotspot mutation mouse model of CSF1R-related disorder.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH antibody Abcam Cat. #ab8245; RRID: AB_2630358 RRID: AB_2630358 Anti-CSF1R R&D Systems Cat. #AF3818; RRID: AB_884158 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (17198) Cell Signaling Technology Cat. #17198S; RRID: AB_2820254 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 488 Anti-Rabbit Recombinant Secondary Antibody (H + L) Invitrogen Cat. #A21206; RRID: AB_2535792 Anti-Neurofilament H Phospho (phos-NFH) Antibody Biolegend Cat. #840801; RRID: AB_2565456 Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 568 Invitrogen Cat. #A10042; RRID: AB_2534017 MBP antibody (F-6) Santa_Cruz Cat. #sc-271524; RRID: AB_10655672 CD11b Monoclonal Antibody (M1/70), APC Invitrogen Cat. #17-0112-83; RRID: AB_469344 AB_ CD45 Monoclonal Antibody (30-F11), FITC Invitrogen Cat. #11-0451-82; RRID: AB_465050 Iba-1 antibody Wako Cat. # 019–19741; RRID: AB_839504 IBA1 antibody guinea pig SYSY Cat. # 234 004; RRID: AB_2493179 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 eBioscience TM (30-F11), PE-Cyanine7, eBioscience TM Invitrogen Cat. # A18710; RRID: AB_2535494, RRID: AB_2535494 AB_ Anti-β-actin antibody Abcam Cat. #ab8226, Cat. #ab8226; RRID: AB_306371 AB_306371 Goat anti-Guinea Pig IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat. #A-21450; RRID: AB_2535867 Chemicals, peptides, and recombinant proteins GM-CSF novoprotein Cat. #CK02 PLX3397 MedChemExpress Cat. #HY-16749 Poly-L-lysine Sigma Cat. #P1274 Bovine Serum Albumin V Solarbio Cat. #A8020 Isoflurane RWD Cat. #R510-22-10 DAPI Yeasen Cat. #40728ES03 RIPA buffer LABLEAD Cat. #R1091 Critical commercial assays RNA isolater Total RNA Extraction Reagent Vazyme Cat. #R401-01 HIScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat. #R333-01 HamQ Universal SYBR qPCR Master Mix Vazyme Cat. #q711-03 Protein Quantification Kit (BCA Assay) Abbkine Cat. #KTD3010-CN SuperKine ECL detection kit Abbkine Cat. #BMU102-CN Biotin-labeled antibodies, antistreptomycin peroxidase MXB Cat. #Kit-9270 DAB Kit MXB Cat. #DAB0031 (Continued on next page) Cell Reports Medicine 7, 102641, March 17, 2026 e1 OPEN ACCESS .. Clinical data from 3 genetically diagnosed probands and their family members who met published criteria for definite CSF1R-RD were obtained from the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University (Medical ethics permission number KY2020-105-02).
Membrane:Article Title: Gasdermin-E mediates mitochondrial damage in axons and neurodegeneration.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (Cat#; RRID) Antibodies Rabbit monoclonal anti-GSDME Abcam #ab215191; RRID:AB_2737000 Rabbit monoclonal anti-GSDMD Abcam #ab209845; RRID:AB_2783550 Rabbit monoclonal anti-Caspase-3 Cell Signaling #9662S; RRID:AB_331439 Mouse monoclonal anti-GAPDH EMD Millipore #CB1001; RRID:AB_2107426 Rabbit monoclonal anti-Cytochrome-C Cell Signaling #11940S; RRID:AB_2637071 Mouse monoclonal anti-ATPF1 Abcam #ab117991; RRID:AB_10901555 Mouse monoclonal anti- ATP5A Abcam #ab14748; RRID:AB_301447 Rabbit polyclonal anti-FLAG Sigma #F7425-.2MG; RRID:AB_439687 Mouse monoclonal anti-Tuj1 Thermo Scientific #MA1-118X; RRID:AB_2536830 Mouse monoclonal anti-GFAP Cell Signaling #3670S; RRID:AB_561049 Rabbit monoclonal anti-Iba1 Cell Signaling #17198S; RRID:AB_2820254 Rabbit monoclonal anti-CD68 Cell Signaling #97778S; RRID:AB_2928056 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling #7074; RRID:AB_2099233 Biotinylated anti-Rb IgG secondary antibody Vector #BA-1000; RRID:AB_2313606 Rabbit monoclonal anti-Isl1/2 Abcam #ab109517; RRID:AB_10866454 Biological samples Sections of human brain tissue, see Table S1 UPenn Center for Neurodegenerative Disease Research Biobank NA Chemicals, peptides, and recombinant proteins Poly-ornithine Sigma #P3655-50mg Laminin Life Technologies #23017-015 Matrigel Basement Membrane Matrix Corning #354234 Neurobasal Medium Gibco #21103 Glutamax Gibco #35050 Dextrose Sigma #D9434-250g MEM NEAA Invitrogen #11140-050 B27 Life Technologies #17504-044 BDNF Peprotech #450-02 GDNF Peprotech #450-13 CNTF Peprotech #450-10 Puromycin Life Technologies #A11138-03 Doxycycline hyclate Sigma #D9891-5g ROCKi (Y-27632) Stemcell Technologies #72304 Raptinal Sigma #SML1745 (Continued on next page) Neuron 111, 1222–1240.e1–e9, April 19, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER (Cat#; RRID) Rotenone Sigma #R8875 Antimycin-A Sigma #A8674 3-NP Sigma #N5636 T-per buffer Thermo #78510 tablet protease inhibitor Sigma #11836153001 HALT protease inhibitor Thermo #87786 1 tablet Phosstop - phosphatase inhibitor tablets Sigma/Roche #04906845001 RIPA lysis buffer EMD Milipore #20-188
Incubation:Article Title: Time-of-day effects on post-exercise phosphoproteomic profiling in mouse hippocampus
Article Snippet: .. Subsequently, the sections were incubated with a primary antibody overnight at 4 °C [1:200 anti-ionized calcium binding adapter molecule 1 (IBA1) antibody, catalog number 17198S, CST; 1:200 mouse anti-glial fibrillary acidic protein (GFAP) antibody, catalog number 80788, CST], and then with a secondary antibody (catalog number GB21301, Servicebio) at room temperature for 1 h. After the last rinsing, the sections were stained with DAPI. ..
Binding Assay:Article Title: Time-of-day effects on post-exercise phosphoproteomic profiling in mouse hippocampus
Article Snippet: .. Subsequently, the sections were incubated with a primary antibody overnight at 4 °C [1:200 anti-ionized calcium binding adapter molecule 1 (IBA1) antibody, catalog number 17198S, CST; 1:200 mouse anti-glial fibrillary acidic protein (GFAP) antibody, catalog number 80788, CST], and then with a secondary antibody (catalog number GB21301, Servicebio) at room temperature for 1 h. After the last rinsing, the sections were stained with DAPI. ..
Staining:Article Title: Time-of-day effects on post-exercise phosphoproteomic profiling in mouse hippocampus
Article Snippet: .. Subsequently, the sections were incubated with a primary antibody overnight at 4 °C [1:200 anti-ionized calcium binding adapter molecule 1 (IBA1) antibody, catalog number 17198S, CST; 1:200 mouse anti-glial fibrillary acidic protein (GFAP) antibody, catalog number 80788, CST], and then with a secondary antibody (catalog number GB21301, Servicebio) at room temperature for 1 h. After the last rinsing, the sections were stained with DAPI. ..
Virus:Article Title: CLN3 mediates chloride efflux from lysosomes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal anti-TFEB Abcam Cat# ab267351; RRID:AB_3674319 polyclonal anti-TFEB Cell Signaling Technology Cat# 4240S; RRID:AB_11220225 monoclonal anti-alpha-tubulin Thermo Fisher Scientific Cat# A11126; RRID:AB_10563441 monoclonal anti-ATP synthase C Abcam Cat# ab181243; RRID:AB_2935765 polyclonal anti-p62 MBL Cat# PM045; RRID:AB_1279301 polyclonal anti-Iba1 FUJIFILM Wako Cat# 019-19741; RRID:AB_839504 monoclonal anti-Iba1 Cell Signaling Technology Cat# 17198S; RRID:AB_2820254 polyclonal anti-GFAP Millipore Cat# AB5541; RRID:AB_177521 monoclonal anti-GFAP Cell Signaling Technology Cat# 3670S; RRID:AB_561049 monoclonal anti-Lamp2 Abcam Cat# AB13524; RRID:AB_2134736 Donkey anti-rabbit Alexa Fluor 647 Thermo Fisher Scientific Cat# A31573; RRID:AB_2536183 Donkey anti-mouse Cy3 Jackson ImmunoResearch Cat# 715-165-151; RRID:AB_2315777 Donkey anti-chicken Alexa Fluor 488 Jackson ImmunoResearch Cat# 703-545-155; RRID:AB_2340375 IRDye® 680RD Goat anti-rabbit IgG (H+L) secondary antibody LI-COR Cat# 926-68071; RRID:AB_10956166 IRDye® 800CW Goat anti-mouse IgG (H+L) secondary antibody LI-COR Cat# 926-32210; RRID:AB_621842 Bacterial and virus strains NEB® Stable Competent E. coli New England Biolabs Cat# C3040H DH5α Competent Cells This paper N/A Chemicals, peptides, and recombinant proteins Hydrochloric acid (HCl) Sigma-Aldrich Cat# 7647-01-0 Methanesulfonic acid (MSA) Sigma-Aldrich Cat# 471356 N-methyl-D-glucamine (NMDG) Sigma-Aldrich Cat# M2004 EGTA Sigma-Aldrich Cat# E4378 HEPES Sigma-Aldrich Cat# H3375 MES hydrate Sigma-Aldrich Cat# M8250 Glucose Sigma-Aldrich Cat# G6152 K-Gluconate Sigma-Aldrich Cat# P1847 KCl Sigma-Aldrich Cat# P9541 NaCl Sigma-Aldrich Cat# S9888 CaCl 2 Sigma-Aldrich Cat# C8106 MgCl 2 Sigma-Aldrich Cat# 208337 NaOH Sigma-Aldrich Cat# 567530 KNO 3 Sigma-Aldrich Cat# 221295 NaNO 3 Fisher Scientific Cat# S343 Ca(NO 3 ) 2 Sigma-Aldrich Cat# C1396 Mg(NO 3 ) 2 Sigma-Aldrich Cat# 237175 NaBr Sigma-Aldrich Cat# 310506 NaI Sigma-Aldrich Cat# 383112 NaH 2 PO 4 Sigma-Aldrich Cat# S3139 K 2 HPO 4 Sigma-Aldrich Cat# P3786 MgSO 4 Sigma-Aldrich Cat# M7506 Ethanol Sigma-Aldrich Cat# 459844 (Continued on next page) Neuron 114, 1–16.e1–e7, March 4, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Fisher Scientific Cat# A452SK-4 2-propanol Sigma-Aldrich Cat# 1.02781 Triton-X 100 Sigma-Aldrich Cat# X100 isoflurane Dechra N/A DMEM Thermo Fisher Scientific Cat# 10566016 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5670701 TrypLETM express enzyme (1X), no phenol red Thermo Fisher Scientific Cat# 12604039 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 MEM amino acids (50x) solution Sigma-Aldrich Cat# M5550 HBSS, no calcium, no magnesium, no phenol red Thermo Fisher Scientific Cat# 14175095 JetOPTIMUS DNA transfection reagent Polyplus Cat# 101000006 Polybrene Sigma-Aldrich Cat# H9268 Puromycin Sigma-Aldrich Cat# P8833 Poly-L-lysine hydrobromide Sigma Aldrich Cat# P1399 Vacuolin-1 Sigma Aldrich Cat# 673000 Nigericin (sodium salt) MedChemExpress (MCE) Cat# HY-100381 Valinomycin Sigma Aldrich Cat# 94675 Tributyltin chloride Sigma Aldrich Cat# T50202 Monensin sodium salt MedChemExpress (MCE) Cat# HY-N0150 Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat# D8418 DIDS Tocris Bioscience Cat# 4523 Niflumic acid Sigma Aldrich Cat# N0630 5-Nitro-2-(3phenylpropylamino)benzoic acid Sigma Aldrich Cat# N4779 Curcumin analog C1 SelleckChem Cat# S6769 Eltrombopag MedChemExpress (MCE) Cat# HY-15306 DTT Thermo Fisher Scientific Cat# P2325 Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1600-100 Normal Donkey Serum Jackson ImmunoResearch Cat# 017-000-121 Protease inhibitor cocktail Sigma Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail I Sigma Aldrich Cat# 4906845001 LDS sample buffer (4x) Thermo Fisher Scientific Cat# NP0008 PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# 23227 Intercept® (TBS) Blocking Buffer LI-COR Cat# 927-60001 20xTBS TweenTM-20 Buffer Thermo Fisher Scientific Cat# 28360 PVDF membrane Sigma Aldrich Cat# IPFL00010 TRIzolTM Reagent Thermo Fisher Scientific Cat# 15596026 RIPA This paper N/A LysoSensorTM Green DND-189 Thermo Fisher Scientific Cat# L7535 Dextran, Oregon GreenTM 488 Thermo Fisher Scientific Cat# D7170 Magic Red Cathepsin L Immunochemistry Cat# 941 DQTM-BSA red dye Thermo Fisher Scientific Cat# D12051 Dil Thermo Fisher Scientific Cat# D282 Critical commercial assays Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S GoTaq® G2 Green Master Mix Promega Cat# M7822 ClonExpress II One Step Cloning Kit Vazyme Cat# C112 2 × Phanta Flash Master Mix (Dye Plus) Vazyme Cat# P520 (Continued on next page) e2 Neuron 114, 1–16.e1–e7, March 4, 2026
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